Protein silver staining.

[He, Xianhui]

I have use this protocol to stain SDS-gel. "But why do you use so high 
concentration of glutaraldehyde(10%)", my colleage in once asked me. So I 
reduce the concentration to 1% or even 0.5% of glutaraldehyde and get the 
same result. By using a shaker and raising temperature, the time for each 
step may be reduced to half of the original and the stain may be finished 
in 2 hour.