Overexpressed E.Coli Protein sticks DNA - help needed!
willis at gandalf.psf.sickkids.on.ca
Fri Mar 8 09:33:52 EST 1996
While I don't have a protocol, I remeber in the old days, we used to
perform a polymine P precipitation of nucleic acids (has the side effect
of taking down any DNA-bound proteins as well).
Also, you could try pre-running your material over a DEAE or DE52 column
to which the nucleic acids will stick gang busters (usually elutes at
Good luck...Randall C Willis, Publisher, Aliquotes Press
"ALIQUOTES: A Journal of Molecular and Biochemical Humour"
58 Balfour Ave.
M4C 1T6 CANADA
416-813-5933 (ph) willis at gandalf.psf.sickkids.on.ca
416-813-5022 (fax) rogerb at microsoft.com
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