Help! Protein Expression in E. coli

[philippe lalle]

Of course it's possible that a protein migrates at an higher molecular 
weight than expected. The most wellknown example is p53 for which the 
theorical molecular weight is something around 43 kD. It depends mainly 
of the amino acid structure: with a lot of prolines the migration is 
slower than expected. I'm now working on a protein that migartes at 190 
kD but its calculated molecular weight is 135kD.
Be optimistic with your data.
Good luck.
Phil