Getting rid of nucleic acids from protein extract

Satish Nair satish at
Wed Mar 13 21:40:09 EST 1996

In article <xma-1203961921330001 at> xma at (Xiao-Jun Ma) writes:
>I need to get rid off nucleic acids from my yeast whole cell extract. I'm
>working with small scale extracts. The nucleic acids present in this
>extract inhibit my enzyme assay. I would appreciate any help on this

Try a 1 to 10 dilution of 5% neutralized polyethyleneamine to crude
lysate... see Burgess, Methods in Enzymology Vol 208 p. 3 for

satish nair

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