Getting rid of nucleic acids from protein extract

Satish Nair satish at a.chem.upenn.edu
Wed Mar 13 21:40:09 EST 1996


In article <xma-1203961921330001 at ts34-2.wla.ts.ucla.edu> xma at ewald.mbi.ucla.edu (Xiao-Jun Ma) writes:
>I need to get rid off nucleic acids from my yeast whole cell extract. I'm
>working with small scale extracts. The nucleic acids present in this
>extract inhibit my enzyme assay. I would appreciate any help on this
>matter.

Try a 1 to 10 dilution of 5% neutralized polyethyleneamine to crude
lysate... see Burgess, Methods in Enzymology Vol 208 p. 3 for
details.

satish nair





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