thrombin for GST prot cleav

[Cornelius Krasel]

GLUXLAB (Laurette at msvax.mssm.edu) wrote:
> 	We are currently using highly purified human thrombin 
> 4,000 Units/mg [sigma #T-3010] for cleaving our fused protein 
> from glutathione S transferase.  the price is becoming 
> prohibitive ~$300/1000 units. is there a better source for
> thrombin, and not neccessarily human that will work as well.
> our end product id being crystallized for structure determination
> as well as for nmr characterization.

I have been told that it is fairly easy to make large amounts of
thrombin from bovine blood but I haven't tried to do it myself.
Generally, less pure preparations may be cheaper but you introduce
additional contaminants and maybe additional proteases.

Good luck!

--Cornelius.

-- 
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: phak004 at rzbox.uni-wuerzburg.de  SP3 */
/* "Science is the game we play with God to find out what His rules are."  */