thrombin for GST prot cleav
krasel at wpxx02.toxi.uni-wuerzburg.de
Wed Mar 13 08:28:26 EST 1996
GLUXLAB (Laurette at msvax.mssm.edu) wrote:
> We are currently using highly purified human thrombin
> 4,000 Units/mg [sigma #T-3010] for cleaving our fused protein
> from glutathione S transferase. the price is becoming
> prohibitive ~$300/1000 units. is there a better source for
> thrombin, and not neccessarily human that will work as well.
> our end product id being crystallized for structure determination
> as well as for nmr characterization.
I have been told that it is fairly easy to make large amounts of
thrombin from bovine blood but I haven't tried to do it myself.
Generally, less pure preparations may be cheaper but you introduce
additional contaminants and maybe additional proteases.
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany email: phak004 at rzbox.uni-wuerzburg.de SP3 */
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