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Isoelectric Points

Bob Hoesch Bob_Hoesch at fws.gov
Thu Mar 14 09:24:21 EST 1996

What kinds of calculations and what sorts of parameters need to be taken into 
consideration in trying to answer the following questions?---

1) What is the largest theoretical isoelectric point shift due to a single AA 
substitution?  (Does anyone know of any observed measurements?)

2) What is the largest apparent pI shift as a result of an AA substitution 
which changes the conformation of the protein but not charge at the individual 
residue position?

Bob_Hoesch at fws.gov
U.S. Fish & Wildlife Forensics Laboratory
Ashland, OR

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