Tris would be fine for binding of the GST fusion protein to the column;
however, the binding of GST to glutathione agarose is better at near
neutral pHs whereas its elution is favored at pH around 8.0. This is
something to keep in mind...
In article <michael.didonato-2802961045160001 at 220.127.116.11>,
michael.didonato at utoronto.ca (Michael DiDonato) wrote:
> I was wondering if it was possible to substitute tris-buffered saline pH
> 8.0 for PBS pH 7.4 in solubilizing GST fusion proteins and applying them
> to the column. My concern is any affect the tris would have on binding of
> the GST fusion to the column.
>> Any help/advice would be greatly appreciated.
> Department of Biochemistry
> University of Toronto
>michael.didonato at utoronto.ca