ligand affinity column preparation

Dima Klenchin klenchin at macc.wisc.edu
Fri Mar 22 11:00:53 EST 1996


In article <4iu3fc$337 at josie.abo.fi>, Tuomo Glumoff <tglumoff at btk.utu.fi> wrote:
->I am trying to prepare a ligand affinity column using Biorad's Affi-Gel 
->102 gel and EDAC coupling chemistry. It doesn't seem to go just like 
->that; it also says in the short procedure supplied with the gel that 
->"reaction pH, reaction time, concentration of EDAC and the coupling 
->solvent can all be varied to optimize ligand coupling".

>Probably the 
->problems vary depending on the ligand, and must be overcome by 
->trial-and-error,
	Yes, very frequently.
->but maybe there are some generally applicable tricks or 
->procedures that would be nice to know? 

It would help if you specify your ligand, conditions and the sort of
problems you encounter. 

- Dima



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