ligand affinity column preparation

Tuomo Glumoff tglumoff at btk.utu.fi
Fri Mar 22 06:38:20 EST 1996


I am trying to prepare a ligand affinity column using Biorad's Affi-Gel 
102 gel and EDAC coupling chemistry. It doesn't seem to go just like 
that; it also says in the short procedure supplied with the gel that 
"reaction pH, reaction time, concentration of EDAC and the coupling 
solvent can all be varied to optimize ligand coupling". Probably the 
problems vary depending on the ligand, and must be overcome by 
trial-and-error, but maybe there are some generally applicable tricks or 
procedures that would be nice to know? Any Affi-Gel Guru willing to 
share the expertise?

Thanks!
Tuomo





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