In article <4ik49k$924 at ipgate.le.ac.uk> "Dr E. Buxbaum" <EB15 at le.ac.uk> writes:
>From: "Dr E. Buxbaum" <EB15 at le.ac.uk>
>Subject: Re: Isolation of nulcei
>Date: 18 Mar 1996 16:51:00 GMT
>I once tried to break up cells by sonication. This left the nuclei
>morphologically intact, of course I don't know if they will still work
>with your experiment. In any case, you need only a few seconds at low
>power to break the cells open (best to work in an ice/water bath).