Proteins which run high on SDS-PAGE

[Matt Parker]

Dilip Dias (v0p8630 at zeus.tamu.edu) writes:
> Matt
> I had the same problem with a protein that I worked and working. When I 
> put the question in the newsgroup the answer I got was that this could 
> happen due to a variety of reasons. But the most important is the amount 
> of prolines that the protein has. Example: p53 protein of which the 
> calculated mol wt is about 45kd?. so be optimistic.
> cheers
> Dilip
> 
> 


	Thanks! Our proteins do have an unusually high amount of proline.
Does anybody have any suggestions about why high proline content would
cause the protein to run "large" on SDS-PAGE?

	By the way...it's not glycosylated. Thanks to all who have sent advice!

	Matt