Concentration of dilute proteins for SDS-PAGE

Dima Klenchin klenchin at macc.wisc.edu
Fri May 3 16:00:38 EST 1996


In article <96124.144926JPS144 at psuvm.psu.edu>,
   Joe Stains <JPS144 at psuvm.psu.edu> wrote:
->Does anyone know of any efficient way of concentrating large volumes of
->a dilute protein (several mls) for use with SDS-PAGE?  I have a couple
->of ideas on some techniques, but would be interested in anything anyone
->else may have, as they all have a lot of pros and cons!

I use centicones after adding SDS to the sample to 0.1% and then
concentrating > 10 fold. After concentration is done, don't forget
to vortex centricon REALLY well. 

- Dima




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