Proteins which run high on SDS-PAGE

[Dilip Dias]

Matt
I had the same problem with a protein that I worked and working. When I 
put the question in the newsgroup the answer I got was that this could 
happen due to a variety of reasons. But the most important is the amount 
of prolines that the protein has. Example: p53 protein of which the 
calculated mol wt is about 45kd?. so be optimistic.
cheers
Dilip