Concentration of dilute proteins for SDS-PAGE

Ron Tate rtate at bmb-fs1.biochem.okstate.edu
Mon May 6 11:58:43 EST 1996


Joe Stains wrote:
> 
> Does anyone know of any efficient way of concentrating large volumes of
> a dilute protein (several mls) for use with SDS-PAGE?  I have a couple
> of ideas on some techniques, but would be interested in anything anyone
> else may have, as they all have a lot of pros and cons!
> 
> Thanks in advance
> 
> Joe Stains

Joe, 
I used to use the TCA method but I've switched to a phenol/ether 
extraction method that I got from -Analytical Biochemistry- vol.226, 
1995, pp.382-383. It works really well.
-- 
>>>>>>--------------------------->
>Ron Tate                                                                   
>Lab of Franklin Leach
>Dept. of Biochem. & Molecular Biology
>Oklahoma State University rtate at bmb-fs1.biochem.okstate.edu
(405) 744-9326
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