Small molecules & nitrocellulose

Dan Brose chemica at bendnet.com
Wed May 8 19:45:10 EST 1996


In article <4mo06d$rae at agate.berkeley.edu>, lhom at nature.berkeley.edu says...
>
>I'm looking to remove proteins from a small volume of a complex biological
>mixture (too small for ultrafiltration, IMHO) and I'm thinking maybe I
>could wet a piece of nitrocellulose and stick it into my sample to do the
>job.  But I'm wondering, what might the nitrocellulose remove besides the
>intended proteins?  
>-- 
>______________________________________________________________________________
_
>Lou Hom >K '93                      "Is that a deliberate act of provocation 
>lhom at nature.berkeley.edu                or just a parochial New Yorkism?" 
>http://www.ocf.berkeley.edu/~lhom        

Is your sample too small to use the small centrifuge vials with UF membranes 
within the centrifuge vial.  I believe you can filter quite small samples by 
this technique.




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