Western Control

Darren Tyson tysondr at sluvca.slu.edu
Fri May 10 16:23:22 EST 1996


rona graham wrote:
> 
> I'm looking for an antibody that i can use on a western to control for
> loading, degradation and transfer of a total protein prep (cytosolic
> fraction). Something like actin is to a northern. any ideas?


It's common practice to simply load the same amounts of total protein
rather than "normalizing" using other Abs.  A positive control of your 
protein of interest is usually sufficient for determining transfer 
efficiencies.

Darren



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