Western Control
Darren Tyson
tysondr at sluvca.slu.edu
Fri May 10 16:23:22 EST 1996
rona graham wrote:
>
> I'm looking for an antibody that i can use on a western to control for
> loading, degradation and transfer of a total protein prep (cytosolic
> fraction). Something like actin is to a northern. any ideas?
It's common practice to simply load the same amounts of total protein
rather than "normalizing" using other Abs. A positive control of your
protein of interest is usually sufficient for determining transfer
efficiencies.
Darren
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