Membrane-impermeable Reducing Agents

[corboy at utdallas.edu]

Michael Daws (Michael.Daws at ucmp.umu.se) wrote:
> I am looking to reduce disulphide bonds outside of cells without 
> affecting internal disulphides.  Is there a reducing agent I can use 
> which is membrane impermeable.  Alternatively is there an oxidising 
> agent that is membrane impermeable?
>  
> Thanks

> Mike Daws

How about glutathione? I don't knoe how much to use, but this is the 
reducing agent used cytoplasmically to prevent disulfide bonds in cells.  
Mike Corboy
University of Texas at Dallas