Immunohistochemistry?

mrtour at stud.med.cornell.edu mrtour at stud.med.cornell.edu
Tue May 14 10:31:32 EST 1996


In article <bilha001-1205961553460001 at dialup-1-22.gw.umn.edu>,
bilha001 at gold.tc.umn.edu (Julaine) wrote:

>    I plan to do immunohistochemistry looking at a nuclear antigen on two
> color FACS sorted cells and cytospun on to a slide. My primary antibody
> for the nuclear antigen is of the same mouse isotype as the primary
> antibody used to sort with the FACS. I predict a cross reaction problem
> when I come back with the secondary Rabbit anti-mouse labled antibody. Any
> quick advice on how to eliminate this background will be appreciated. 
>      A reply by email is fine bilha001 at gold.tc.umn.edu

protease treatment?

michelle

-- 
db

or Michelle



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