codon usage chart needed

Tom Duncan duncant at
Tue May 14 10:14:39 EST 1996

Penny Avoles wrote:
> I am looking for information on codon usage within E.Coli to try to 
> improve the expression of a eukaryotic gene.  Please provide me with a
> reference or a chart itself.  Thanks for your help.
> Penny

	Codon usage summaries are published every few years in the journal
Nucleic Acids Research. The latest I have on hand is NAR 16 (1988),
r315-r402 (supplement). There are several codons in E. coli that may
cause expression problems, but success does not always correlate with a
change to a more frequently-used codon.

	Good luck,

	Thomas M. Duncan
	Dept. Biochemistry & Molecular Biology
	SUNY Health Science Center
	750 E Adams St,	Syracuse, NY 13210
	Email:	duncant at or
		duncant at

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