staining a chargeless protein
oandersn at pw.usda.gov
Tue May 14 19:19:25 EST 1996
We are working with a 55 kDa polypeptide that has no charged amino acids.
Attempts to stain the polypeptide on PAGE gels has failed - tries
included commasie blue, amido black, silver, copper stain, Sypro,
anilionaphthalene sulfonate, and fluorescein isothiocyanate. The only
amino group is the N-terminal and it seems unreactive/blocked. The
only amino acids in the polypeptide are P, G, Q, Y, T, and S. Any
suggestions how to stain/detect this polypeptide in a gel?
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