site-directed mutagenesis

Flip Hoedemaeker fhoedem at oci.utoronto.ca
Wed May 15 08:59:14 EST 1996


I want to do site-directed mutagenesis to introduce a restriction site
in the gene I'm expressing. It's been a while since I did this kind of
thing, so I'm wondering what the best SDM kits are at the moment.
Preferrably, I like to avoid recloning of my construct, i.e. there must
be kits that work on expression vectors (pET for example). Can you
please tell me which kit you use, and what your rate of success is.

Thanks, Flip



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