staining a chargless protein

Shaun D. Black SHAUN at JASON.UTHCT.EDU
Thu May 16 19:23:59 EST 1996


Hi All,

     Gee, this is a fascinating problem.  I don't guarantee that it would
work, but you might try the zinc-imidazole stain.  It is almost as sensitive
as Coomassie but is only stable for about 6 hours.  Thus, you must photograph
the gel to have a permanent record.  Check it out in:
     
     Fernandez-Patron, C. et al. Biotechniques 12(4): 564-573 (1992)
     "Reverse Staining of Sodium Dodecyl Sulfate Polyacrylamide Gels
     by Imidazole-Zinc Salts: Sensitive Detection of Unmodified Proteins".

Have fun...   -Shaun

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  = Shaun D. Black, PhD   | Internet address:     shaun at jason.uthct.edu = 
  = Dept. of Biochemistry | University of Texas Health Center, at Tyler = 
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