staining a chargeless protein
102063.2640 at CompuServe.COM
Sat May 18 21:39:34 EST 1996
How about staining the gel rather than the protein? There are
numerous reverse- or negative-staining methods about, but the one I
use (and like a whole lot for preparative purposes) is the zinc
method of Fernandez-Patron et al. [Fernandez-Patron, C.,
Castellanos-Serra, L. and Rodriguez, P. (1992). Reverse staining of
sodium dodecyl sulfate polyacrylamidde gels by imidazole-zinc salts:
Sensitive detection of unmodified proteins. Biotechniques
12:564-573]. Best of luck.
The opinions expressed above are recent, are are unlikely to
coincide with those of my employer.
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