CTAB and Membrane Protein Purification

Yves Bertheau bertheau at solair.inapg.inra.fr
Sat May 18 09:37:58 EST 1996


Federici wrote:
> 
> In article (Dans l'article) <3187492D.3FDC at igmors.u-psud.fr>, Mark Blight
> <BLIGHT at igmors.u-psud.fr> wrote (écrivait) :
> 
> >         =
> >
> >    Tel: (+33 1) 69 41 66 99
> >                         Fax: (+33 1) 69 41 78 08
> >    e-mail:      blight at igmors.u-psud.fr
> > ____________________________________________
> 

> used in this purpose. The final content of CTAB is determined by: nucleic
> acids/CTAB=1 (W/W).This rate must be respected, because an excess of CTAB
> would react with SDS and hinder a following SDS-PAGE. (you must know that


More precisely, the effect of the CTAB is depending on the salt (e.g.
NaCl) concentration. This property is used in several protocol
to extract DNA from plants, fungi and bacteria. Depending on the salt
concentration, you will let resuspend either proteins  or DNA.

Moreover, CTAB is commonly used as a disinfectant according to its
solubilizing (i.e. lyze of bacteria) properties. In France: cetavlon.


-- 
Yves Bertheau, INRA INA P-G, Pathologie Vegetale, 16 rue Claude Bernard,
75231 PARIS cedex 05, FRANCE, Tel +33 (1) 44.08.16.98  or 44.08.17.04
Fax: +33 (1) 44.08.17.00 or 16.31, Internet:
bertheau at inapv.inapg.inra.fr
http://inapv.inapg.inra.fr/Welcome.html



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