western blot of lipoproteins

sylvie barray sylvie.barray at crihan.fr
Wed May 22 03:16:59 EST 1996


I am trying to detect a putative bacterial lipoproteic endoglucanase by 
western blot (after SDS-PAGE) with a polyclonal antibody on nitrocellulose 
membran. The antibody recognize  the  protein only  when it is deleted of 
NH2ter domain (putative lipidic motif). 
thank you for yours suggestions.
sylvie.barray at coloris.univ-rouen.fr



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