codon usage chart needed

Peter Wang plw at mrc-lmb.cam.ac.uk
Wed May 22 17:36:32 EST 1996


Tom Duncan wrote:
> Penny Avoles wrote:
> > I am looking for information on codon usage within E.Coli to try to
> > improve the expression of a eukaryotic gene.  Please provide me with a
> > reference or a chart itself.  Thanks for your help.
> > Penny
> 
>         Codon usage summaries are published every few years in the journal
> Nucleic Acids Research. The latest I have on hand is NAR 16 (1988),
> r315-r402 (supplement). There are several codons in E. coli that may
> cause expression problems, but success does not always correlate with a
> change to a more frequently-used codon.

There is a more recent 1991 NAR compilation in Vol. 19 (Supplement) p. 1981.
Also, there is a web site with compilations of codon usage:
     http://www.dna.affrc.go.jp/~nakamura/CUTG.html

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Peter Wang, M.D., Ph.D.
MRC Centre for Protein Engineering,
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