Protein Elution from SDS Gels

Ron Tate rtate at bmb-fs1.biochem.okstate.edu
Wed May 22 15:19:23 EST 1996


Tom Deits wrote:
> 
> We have just a few protein samples which we would like to elute from SDS PAGE
> gels for subsequent analysis.  If you have a good method that doesn't require
> purchase of an electroeluter, please let me know.
> 
> Thanks
> 
> -Tom Deits
> MBI International
> deits at mbi.org

Depending on how you want to further analyze them you can blot them onto 
PVDFor nylon, or cut out the bands and use a procedure of Amicons with 
their spin-concentrators to elute them into buffer. 
Happy Hunting
-- 
>>>>>>--------------------------->
>Ron Tate                                                                   
>Lab of Franklin Leach
>Dept. of Biochem. & Molecular Biology
>Oklahoma State University rtate at bmb-fs1.biochem.okstate.edu
(405) 744-9326
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