Protein Elution from SDS Gels
Ron Tate
rtate at bmb-fs1.biochem.okstate.edu
Wed May 22 15:19:23 EST 1996
Tom Deits wrote:
>
> We have just a few protein samples which we would like to elute from SDS PAGE
> gels for subsequent analysis. If you have a good method that doesn't require
> purchase of an electroeluter, please let me know.
>
> Thanks
>
> -Tom Deits
> MBI International
> deits at mbi.org
Depending on how you want to further analyze them you can blot them onto
PVDFor nylon, or cut out the bands and use a procedure of Amicons with
their spin-concentrators to elute them into buffer.
Happy Hunting
--
>>>>>>--------------------------->
>Ron Tate
>Lab of Franklin Leach
>Dept. of Biochem. & Molecular Biology
>Oklahoma State University rtate at bmb-fs1.biochem.okstate.edu
(405) 744-9326
---------------------------------------------
More information about the Proteins
mailing list