biotin-avidin binding

Keld Sorensen KeldS at uic.edu
Wed May 22 18:49:06 EST 1996


Nonspecific binding could be a problem. Try the new 
NeutrAvidin-HRP conjugate from Pierce (prod 31001).
NeutrAvidin is simply de-glycosylated avidin, and has
inherently lower non-specific binding, mainly due to the
neutral isoelectric point.

On the other hand, you might be seeing endogenously 
biotinylated proteins!!! In which case you CAN NOT
use avidin-HRP since you would pick up those bands.

Keld .



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