Protein separations using Ion Chromatography

IAN MCFARLANE imcfarlane at its.dundee.ac.uk
Mon May 27 04:44:31 EST 1996


In article <31A5F63C.1C12 at rutchem.rutgers.edu> hall at RUTCHEM.RUTGERS.EDU ("G.S. Hall") writes:
>From: hall at RUTCHEM.RUTGERS.EDU ("G.S. Hall")
>Subject: Protein separations using Ion Chromatography
>Date: 24 May 1996 07:50:43 -0700

>We want to separate proteins, albumin, cerulloplasmin, transferrin, and 
>myoglobulin from human serum.  Tried GPC but resolution can not separate 
>transferring and albumin.  I heard that ion chromatography using 
>gradients works well.  What type of column would you recommend?


Have you thought about dye-affinity chromatography. Pharmacia sell a HiTrap 
Blue column which is supposed to separate different serum proteins.
Try Gianazza, Biochem. J., 1982, v203, p637-641

Ian Mc



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