help with circular dichroism
alvin at cs.mun.ca
Mon May 27 12:15:33 EST 1996
To anyone who can help,
I have been using circular dichroism to study the thermal
stability of an à-helical protein. When the unfolding data is
plotted as: (1) relative ellipticity at 222 nm versus T and
(2) relative ellipticity at 208 nm versus T, the profiles are
somewhat similar but not identical. What is the reason for the
difference ? What electronic transitions do the minima at 208 nm
and 222 nm correspond to ?
I am using a Jasco- J-500 A. The records are from sixteen repeats
If you can provide any information you feel may be helpful it
would be greatly appreciated.
Thank you very much,
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