dye migration on PAG

Nicholas Plummer nplummer at umich.edu
Tue May 28 15:01:07 EST 1996

I'm attempting my first protein eletrophoresis and western blots, and I
have a very basic question on dye migration:

I'm using a 6% polyacrylamide gel.  The bisacrylamide to acrylamide ratio
is 1:29.  I would like to know the approximate mass of protein that
co-migrates with the bromophenol blue dye front on such a gel.  Can any
point me to a reference that might give this information for varying gel
concentrations or crosslinker rations?



Nicholas Plummer
nplummer at umich.edu

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