dye migration on PAG
Nicholas Plummer
nplummer at umich.edu
Tue May 28 15:01:07 EST 1996
I'm attempting my first protein eletrophoresis and western blots, and I
have a very basic question on dye migration:
I'm using a 6% polyacrylamide gel. The bisacrylamide to acrylamide ratio
is 1:29. I would like to know the approximate mass of protein that
co-migrates with the bromophenol blue dye front on such a gel. Can any
point me to a reference that might give this information for varying gel
concentrations or crosslinker rations?
Thanks,
Nick
--------------------------
Nicholas Plummer
nplummer at umich.edu
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