Backgound problems in Western blotting - HELP!

Med. Gas. Lab. mgl5403 at
Tue May 28 09:38:11 EST 1996

Hi netters

   I am doing Western blotting and using the ECL-detection system. My
problem is that I gt an irregular, uneven background. I have tried
modifying washing procedures and blocking buffers.
   I am using the PHAST-system from Pharmacia. Does anyone out there
have an absolutely super protocol, that I can try out??


Jakob Hendel
 Jakob Hendel
  Med Gas Lab 5403
    Kas Herlev
Phone: +45 44535330*3421
Fax:   +45 44535332
e-mail: jhendel at 

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