electroelution of proteins

[ijiwaru at wheel.dcn.davis.ca.us]

In article <4ohnt7$g0q at lyra.csx.cam.ac.uk>, cdw1000 at mole.bio.cam.ac.uk
(Christopher Walentas (Bioc)) wrote:

> Hi.
> 
> I was wondering if anyone out there could recommend a protocol for
>  electroelution of proteins from an SDS-PAGE.  I've got two proteins
>  that will only separate on an SDS gel, and now I want to try and
> get them back out again.  Things like getting rid of the SDS and
> COomassie and THEN refolding in a controled manner (from say urea)
> would be of great help.  If there is anyone with experience/ luck
> in this field, I would be grateful if they would contact me.
> 
> Cheers!
> 
> Tripp

Tripp,

you might want to check out Hager and Burgess (1970) Anal. Biochem.
109:76-86.  They describe a method for KCl staining of proteins that might
be easier to get rid of than the coomassie.  I've used their method for a
protein that I was interested in and had pretty good luck.  The only thing
is they use passive elution, but I'm sure that it should work with
electroelution as well.

Good luck,

Lyle Najita
Plant Pathology
University of California - Davis