Backgound problems in Western blotting - HELP!

Federici federici at icgm.cochin.inserm.fr
Fri May 31 04:38:35 EST 1996


In article (Dans l'article) <4of00m$uh at underworld.centrum.dk>,
mgl5403 at centrum.dk (Med. Gas. Lab.) wrote (écrivait) :

> Hi netters
> 
>    I am doing Western blotting and using the ECL-detection system. My
> problem is that I gt an irregular, uneven background. I have tried
> modifying washing procedures and blocking buffers.
>    I am using the PHAST-system from Pharmacia. Does anyone out there
> have an absolutely super protocol, that I can try out??
> 
> Greetings
> 
> Jakob Hendel
> ________________________   
>  Jakob Hendel
>   Med Gas Lab 5403
>     Kas Herlev
> Phone: +45 44535330*3421
> Fax:   +45 44535332
> e-mail: jhendel at ibm.net 
> __________________________
are you sure the observed background is not due to a bad wrapping of the
ECL treated membrane? The membrane must be located between two
well-stretched pieces of barrier food wrap.



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