Reprobing western blots
The Kindly One
don't at worry.about.it
Sat Nov 2 23:39:27 EST 1996
Biol. Cel. <bc2biced at lucano.uco.es> wrote in article
<327545E7.6162 at lucano.uco.es>...
> I need to reprobe western blots with a primary antibody after
> detect with ECL a firs immunodetection. How can I separate the first
> antibody and reprobe the blot?
> Thank you in advance.
Read the protocol given by ECL!!! It works great for us. I think it
involves using 2X tris and
incubating for an hour or so at 50(or 60) degrees. That should take off the
primary Ab and then
you can reprobe. It's all in the ECL booklet.
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