immobilized neuraminidase

[Tom Vink]

hornig at bc.biol.ethz.ch (Ruediger Hornig) wrote:

>Hello everybody,

>for removing sialic acid residues from the outside of intact cells, I
>would like to use neuraminidase. Does anybody know who is supplying it
>immobilized on beads or does anybody think of a different way to remove
>the enzyme from the reaction mixture ?

>thanks in advance for your help

>Rudi

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Dear Rudi,

You could inactivate the Neuraminidase by adding an inhibitor after
the treatment of the cells.