immobilized neuraminidase

Tom Vink tvink at xs4all.nl
Mon Nov 4 06:07:31 EST 1996


hornig at bc.biol.ethz.ch (Ruediger Hornig) wrote:

>Hello everybody,

>for removing sialic acid residues from the outside of intact cells, I
>would like to use neuraminidase. Does anybody know who is supplying it
>immobilized on beads or does anybody think of a different way to remove
>the enzyme from the reaction mixture ?

>thanks in advance for your help

>Rudi

>----------------------------------------------------------------------
>    _/_/_/_/_/_/_/_/_/    _/    ETH Zurich
>   _/        _/    _/    _/    Biochemie II
>  _/_/_/    _/    _/_/_/_/    Rüdiger Hornig (hornig at bc.biol.ethz.ch)
> _/        _/    _/    _/    Universitaetstr. 16, ETH Zentrum
>_/_/_/_/  _/    _/    _/    CH-8092 Zurich (Switzerland)
>                           Phone: +41 1 632 3007  FAX: +41 1 632 12 69

>         http://www.bc.biol.ethz.ch/BiochemistryII/BioII.html
>----------------------------------------------------------------------
Dear Rudi,

You could inactivate the Neuraminidase by adding an inhibitor after
the treatment of the cells.






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