TCA precipitation

Jeff Cohlberg cohlberg at csulb.edu
Fri Nov 8 12:35:52 EST 1996


Eva Dupille (poseva at iata.csic.es) wrote:
: Hello,
: I have a protein problem precipitation. I did a TCA precipitation, 15 % =
: for 1 h at 4 C, and I got, nothing at all.

The efficiency of TCA precipitation often depends on salt concentration.
If you're trying to precipitate from a dilute buffer, try adding 0.2 M
NaCl before adding the TCA.
 -- 
Jeffrey A. Cohlberg, Professor
Department of Chemistry and Biochemistry
California State University, Long Beach
1250 Bellflower Boulevard, Long Beach, CA 90840
Phone: (310) 985-4944	FAX: (310) 985-8557   E-mail: cohlberg at csulb.edu



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