Ni column problem

Panomporn Panutat ppanutat at CENTRAL.MURDOCH.EDU.AU
Mon Nov 11 05:25:18 EST 1996

I have a problem with eluting protein from Ni-column (QIAGEN) . I 
followed spin column protocol described by the manufacturers but the 
protein remains bound to the column. Does anyone have any suggestions as 
to how I can elute my protein without denaturing it.
Any suggestions greatly appreciated.

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