Specificity of Endoprot
Marcus, Dr J.
J.Marcus at botany.uq.edu.au
Mon Nov 11 23:30:41 EST 1996
In an earlier post I suggested using Iodoacetamide to
modify Cys residues to make them susceptible to Trypsin
cleavage. I was quite mistaken, as Daniel Milligan kindly pointed out
to me. Shaun Black's suggestion to use the ethyleneimine is the
suggestion that I should have made. Sorry if I caused any
> Date: 11/11/96 4:13 PM
> To: protein-analysis at net.bio.net
> From: Marcus at tpp.uq.edu.au
> Dear Kali,
> You wrote:
> > I am looking for something to specifically cleave at Cys residues. I
> > am aware of Endoproteinase Asp-N which cleaves at Aspartic acid also.
> > Does anyone know of anything I could use? I am looking forward to your
> > great ideas.
> > Thanks for the help, Kali
> If you are really desparate you might try modifying Cys
> residues with iodoacetamide. Once modified, I think it
> allows trypsin to cleave at that position (maybe not
> efficiently but you should get some cleavage). You would
> have to modify both arginines and lysines (see Pierce
> catalog) so that they are not susceptible to trypsin
> cleavage. A bit of work, but if you are desparate, it
> might work.
> Hope that helps.
John Marcus Marcus at tpp.uq.edu.au (Dr J.Marcus)
Cooperative Research Centre for Tropical Plant Pathology
5th Level John Hines Building
University of Queensland
St. Lucia, QLD 4072
More information about the Proteins