small volume dialysis

[Ron Tate]

kewugator at UFCC.UFL.EDU wrote:
> 
> Hi, there:
> I am going to dialysis some proteins (30 KDa) in very small volume (20 ul).
> Any body has any idea that how can I have a good recovery of the proteins and
> what kind of dialysis apparatus can be used to get the maximum recovery of the
> proteins.
> Thanks.
> 
>                                                 Ke Wu

I posted this suggestion a month or so ago also, BioTechniques published
a way to do this last year, Vol. 19, No.2 (1995) (I don't have the page
number) the article was titled "Waterbug Dialysis".  It involved cutting
the top portion off of a microfuge tube and closing a piece of dialysis
membrane in the lid with your sample inside the lid and then floating
the thing in a beaker of buffer.  Its one of those "Why didn't I think
of that" kind of ideas.  
If you don't have access to back issues of BioTechniques they are online
with their back issues and a searchable d-base.
Happy Hunting
-- 
>>>>>>--------------------------->
Ron
Tate                                                                   
Lab of Franklin Leach
Dept. of Biochem. & Molecular Biology
Oklahoma State University 
rtate at bmb-fs1.biochem.okstate.edu
(405) 744-9326
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