Ni column problem
qiagen at kaiwan.com
Thu Nov 14 03:34:30 EST 1996
ppanutat at CENTRAL.MURDOCH.EDU.AU (Panomporn Panutat) wrote:
>I have a problem with eluting protein from Ni-column (QIAGEN) . I
followed spin column protocol described by the manufacturers but the protein
remains bound to the column. Does anyone have any suggestions as to how I can
elute my protein without denaturing it. Any suggestions greatly appreciated.
There are many possible reasons why there was no protein in your eluate
from the Ni-NTA spin column, however it is not possible to advise you
without access to further information. Please call QIAGEN Technical
Services. In Australia, QIAGEN Pty Ltd can be reached at (03) 9489 3666.
We would be happy to share our experience with you, and do our best to
help you successfully purify your protein under non-denaturing conditions.
Temporary e-mail address: qiagen at Kaiwan.com
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