TCA precipitation

Federici federici at icgm.cochin.inserm.fr
Thu Nov 14 08:47:01 EST 1996


In article (Dans l'article) <01BBCD5C.B2A71400 at alzacarias.iata.csic.es>,
poseva at iata.csic.es (Eva Dupille) wrote (écrivait) :

> Hello,
> I have a protein problem precipitation. I did a TCA precipitation, 15 % =
> for 1 h at 4 C, and I got, nothing at all.
> Sure that I have proteins, I get enzyme activity and with biorad, before =
> precipitation, I have around 10 micrograms/ml.
> Is it a problem of temperature or incubation time ??
> Please, if you have some comments, let me know. That s boring to lose so =
> many time in protein purification, to lose all the samples for a protein =
> precipitation problem ..
> Thanks in advance, sorry for the approximative english.
> Eve

why don't you carry out precipitation on a more concentrated solution ?



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