Protein ultrafiltration query
jayuziuk at rs5.tcs.tulane.edu
Fri Nov 15 12:29:38 EST 1996
Are you using cellulose based membranes? You may have a cellulase present.
Do standard proteins of similar size pass through the membranes before or
after are used for your enzyme?
Haralt Leiter (hleiter at edv2.boku.ac.at) wrote:
: Sorry, I earlier forgot a subject line:
: Hi everybody !
: I´m a poor PhD Student (my supervisor hasn´t paid me) purifying a
: fucosyltransferase with an estimated (SDS-PAGE) molecular weight of at
: least 50kDa. During ultrafiltration the entire enzyme-activity passes
: through the membrane (cutoff 30000). Has anybody observed a similar
: effect or can offer a reasonable explanation ?
: I use a 25mM Tris/HCl pH7,3
: 0,1% Triton X-100
: 10% Glycerol
: 0,02% NaN3 buffer and the applied protein solution contains
: hundreds of micograms.
: Thanks for any answers in advance
: BTW, I have already tried membranes of different companies
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