Ni column problem
Achim Recktenwald, PhD
achim at ibex.ca
Fri Nov 15 07:47:12 EST 1996
> ppanutat at CENTRAL.MURDOCH.EDU.AU (Panomporn Panutat) wrote:
> >I have a problem with eluting protein from Ni-column (QIAGEN) . I
> followed spin column protocol described by the manufacturers but the protein
> remains bound to the column. Does anyone have any suggestions as to how I can
> elute my protein without denaturing it. Any suggestions greatly appreciated.
> Dear Panomporn,
> There are many possible reasons why there was no protein in your eluate
> from the Ni-NTA spin column, however it is not possible to advise you
> without access to further information. Please call QIAGEN Technical
> Services. In Australia, QIAGEN Pty Ltd can be reached at (03) 9489 3666.
> We would be happy to share our experience with you, and do our best to
> help you successfully purify your protein under non-denaturing conditions.
> QIAGEN, Inc.
> Temporary e-mail address: qiagen at Kaiwan.com
One possible problem could be the size of the protein. The tech. support
once told me the spin columns are only good for small proteins (sorry,
cannot remember a number). For large proteins you have to make your own
small column from the loose gel.
More information about the Proteins