Ni column problem

Achim Recktenwald, PhD achim at
Fri Nov 15 07:47:12 EST 1996

QIAGEN wrote:
> ppanutat at CENTRAL.MURDOCH.EDU.AU (Panomporn Panutat) wrote:
> >I have a problem with eluting protein from Ni-column (QIAGEN) . I
> followed spin column protocol described by the manufacturers but the protein
> remains bound to the column. Does anyone have any suggestions as to how I can
> elute my protein without denaturing it. Any suggestions greatly appreciated.
> >Thanks
> >Panomporn
> Dear Panomporn,
> There are many possible reasons why there was no protein in your eluate
> from the Ni-NTA spin column, however it is not possible to advise you
> without access to further information. Please call QIAGEN Technical
> Services. In Australia, QIAGEN Pty Ltd can be reached at (03) 9489 3666.
> We would be happy to share our experience with you, and do our best to
> help you successfully purify your protein under non-denaturing conditions.
> --
> QIAGEN, Inc.
> 1-800-426-8157
> Temporary e-mail address: qiagen at

One possible problem could be the size of the protein. The tech. support
once told me the spin columns are only good for small proteins (sorry,
cannot remember a number). For large proteins you have to make your own
small column from the loose gel.


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