Streaking on nitrocellulose and PVDF membranes

[Jeffrey Yuziuk]

I get the same thing! I'm using 4-chloro-napthol to visualize. One thing I
noticed is that I don't have the problem when I don't use reducing agent
in my sample buffer. As a matter of fact, when I load half the gel with
sample containing 2-ME or DTT, and the other half of the same gel with the
same sample minus the reducing agents, I get streaks only on the half of
the gel with the reducing agent, but not on the side without. I also tried
cleaning the glass ware...

Jeff



Demeke, Tigst (tdemeke at pbi.nrc.ca) wrote:
: 
: I consistently get streaks on my western blots....(etc.)