HLEITER at EDV2.BOKU.AC.AT
Mon Nov 18 10:11:55 EST 1996
Date: Fri, 15 Nov 1996 09:29:10 -0700
To: hleiter at edv2.boku.ac.at
From: Hiranya Roychowdhury <hroychow at nmsu.edu>
Subject: Re: (no subject)
Cc: protein-analysis at net.bio.net
Just a possibility:
A polypeptide showing an Mr or 50kDa on SDS-PAGE may very well have a
physical Skokes radius equivalent to 30kDa (or slightly less) in its native
form. Also, check the retention using conventional dialysis using membranes
of 30k (and less) cut-offs.
Dr. Hiranya Sankar Roychowdhury
Plant Genetic Engineering Lab.
New Mexico State University
Las Cruces, NM 88003
Ph. (505) 646-5785
hroychow at nmsu.edu
Thank you for your help - I can only imagine what the Skokes radius
is - I couldn`t find it in a book - can you give me a hint ?
The same effect using dialysis membranes - only a cutoff of 10000MW
gives good results.
+ Haralt Leiter Institut fuer Chemie +
+ e-mail: leiter at edv1.boku.ac.at Univ. f. Bodenkultur +
+ Tel.: +43-1-36006-6065 Muthgasse 18 +
+ +43-1-36006-6511 (Lab) 1190 Wien - Austria +
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