Streaking on nitrocellulose and PVDF membranes

Demeke, Tigst tdemeke at
Mon Nov 18 09:13:07 EST 1996

I consistently get streaks on my western blots.  Some of the streaks start 
from top and continue to the bottom of the gel or stop somewhere in the 
middle, while others start from the middle or even lower and continue for 
either long distance or short distance.  I have tried the following: 
decreasing the amount of protein loaded, reducing the current, lowering the 
concentration of primary and secondary antibodies, filtering and degassing 
of acrylamide/bisacrylamide solution, filtering the loading dye, thoroughly 
cleaning the plates with detergent and water, washing the wells with DDH2O. 
 I still get streaks.  Do you have any suggestions to get rid off the nasty 
Tdemeke at

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