Need help with GST fusion protein purification

Dr E. Buxbaum EB15 at le.ac.uk
Mon Nov 18 13:39:25 EST 1996


If everything else fails, forget about your tag and do conventional 
protein purification. I was in that situation once when a MBP-conjugate 
absolutely refused to bind to the affinity column. 

Ammoniumsulfate fractionisation, gel chromatography and ion exchange 
chromatography gave me a pure product and a good yield. Not very elegant, 
but it worked.




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