Need help with GST fusion protein purification
Dr E. Buxbaum
EB15 at le.ac.uk
Mon Nov 18 13:39:25 EST 1996
If everything else fails, forget about your tag and do conventional
protein purification. I was in that situation once when a MBP-conjugate
absolutely refused to bind to the affinity column.
Ammoniumsulfate fractionisation, gel chromatography and ion exchange
chromatography gave me a pure product and a good yield. Not very elegant,
but it worked.
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