secretory protein->Try pSecTag

Bradley Turner bsturner at MBCRR.HARVARD.EDU
Tue Nov 19 15:38:26 EST 1996


Hello Jaswant,

You may want to try the pSecTag A,B,C vector from InVitrogen.  It has a
number of the features that you seem to want. i.e.:

a CMV (cytomegalovirus) promoter for protein expression
an Igk leader sequence which directs fusions into the secretory pathway
a MCS (multiple cloning site) available  in three different reading frames
a myc epitope for detection with antibodies
a 6-histidine tail for nickel column purification of fusion peptides
a BGH (bovine growth hormone) polyA signal site
a Zeocin (bleomycin)resistance gene for selection of stable transfectants
an Ampicillin resistance gene for selection in ecoli
a pUC ColE1 origin for replication in ecoli

It is catalog number V900-20

Invitrogen Corp
3985 B Sorrento Valley Blvd
San Diego CA 92121
Tel: 800-955-6288
fax: 619-597-6201
email: tech_service at invitrogen.com
http://www.invitrogen.com

I think you can download the sequence from their web site or they can
fax or email it to you.

I hope this helps ,
Brad Turner,

Disclaimer: No commercial ties, just a satisfied customer.

**************************************************************
		    Bradley Turner
	        Beth Israel Deaconess
                    Medical Center

Harvard Medical School		617-667-1215 phone
Division of Gastroenterology	617-667-2767 fax
Room Dana 536			bsturner at mbcrr.harvard.edu
330 Brookline Avenue		bturner at bidmc.harvard.edu
Boston, MA 02215		turner at sprcore.bidmc.harvard.edu
**************************************************************



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