Q: Checking DNA purity using spec...

Otter otterpop at pilot.msu.edu
Tue Nov 19 16:54:49 EST 1996


I have been using the standard protocol to determine isolated DNA purity by
measuring the light absorbance at 260 and 280 nm.  I know that pure DNA
absorbs at a wavelength of 260 and protein at a wavelength of 280.  My
questions is what exactly or specifically is absorbing the light at those
wavelengths (i.e. does one base absorb more light than another at 260, are
there specific amino acids that are absorbing at 280 etc...)?

Thanks for your time and help,

Otter



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